Details

Microscopic Techniques in Biotechnology

1. Aufl.

von: Michael Hoppert
126,99 €
Verlag:	Wiley-Blackwell (WB)
Format:	PDF
Veröffentl.:	06.03.2006
ISBN/EAN:	9783527605231
Sprache:	englisch
Anzahl Seiten:	342

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Beschreibungen

Titelbeschreibung

Focusing on all current applications, this book presents the various methods as well as their suitability and limitations for a specific question. One particular highlight is the presentation of all basic information on the structure of the relevant objects, thus allowing readers to choose the most suitable applications for any specific problem. They will also find in-depth background information on structure-function relationships, plus descriptions of sample preparations with respect to a particular technique and the necessary equipment. The whole is rounded off with an overview of the future application potential for devices and applications of upcoming interest in biotechnology.

Inhaltsverzeichnis

Preface PART 1: LIGHT MICROSCOPY CONVENTIONAL LIGHT MICROSCOPY AND STEREOMICROSCOPY Introduction: Applications and Limitations in Light Microscopy Useful Applications Preparation Techniques CONFOCAL LASER SCANNING MICROSCOPY (CLSM) Introduction: Applications and Limitations in CLSM Useful Applications Preparation Techniques PART 2: ELECTRON MICROSCOPY SCANNING ELECTRON MICROSCOPY Introduction: Applications and Limitations in Scanning Electron Microscopy Useful Applications Preparation Techniques TRANSMISSION ELECTRON MICROSCOPY Introduction: Applications and Limitations Useful Applications Preparation Techniques PART 3: OTHER MICROSCOPICAL METHODS AND THEIR APPLICATION POTENTIAL SCANNING PROBE MICROSCOPY Atomic Force Microscopy Near Field Scanning Optical Microscopy (NSOM) ACOUSTIC MICROSCOPY SPECIAL MICROANALYTICAL METHODS CONCLUDING REMARKS References

Rezension

"...conceptual and stylistic homogeneity...sound textbook that can give fundamental insights and be accompanying own practical work in biology." (ChemPhysChem, Vol. 4, No. 12, Petra Schwille)

Autorenportrait

Michael Hoppert is the author of Microscopic Techniques in Biotechnology, published by Wiley.

Back cover copy

For years, microscopy has proven to be an invaluable tool for all fields of biological and medical research. But not only at the level of a laboratory experiment, also after scale up, in all fields of biotechnological processes the microscope is a routine tool for the biologists, the engineer of the technician. Analysis of a visual image of a cell or cellular constituent, a biofilm or a tissue sample allows – at one instance – exploration of a variety of important features such as total quantity of the objects, cell volume and volume of compartments, presence of contaminants, or infective agents and status of the cell cycle. Numerous straightforward and standardized techniques detect and identify cellular organelles, specific nucleotide sequences, proteins, polymers and products of low molecular weight. In introducing sections of this book, the practicizing biotechnologist, who may not necessarily be trained in biology, will find illustrative examples for the possibilities of a certain microscopic technique. At the same time, he is introduced into the structural features of a biological specimen, a subcellular compartment, a cellular tissue or a biofilm, as exemplified by a certain preparation and imaging technique. In a respective methods section, the user is introduced into instrumentation and will find a selection of straightforward preparation techniques, including short general descriptions and step-by-step protocols. This presentation allows a quick start of the user for his own experiment.