Details
In Situ Hybridization
Laboratory Companion1. Aufl.
43,99 € |
|
Verlag: | Wiley-Blackwell (WB) |
Format: | |
Veröffentl.: | 12.06.2008 |
ISBN/EAN: | 9783527615063 |
Sprache: | englisch |
Anzahl Seiten: | 183 |
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Beschreibungen
In situ hybridization is a proven, powerful technique with applications in chromosome and genome analysis, as well as gene expression. Covering a carefully selected range of techniques with immediate and general applications in research and clinical diagnosis, the book starts with genome and DNA mapping, continues through gene expression localization in wholemount and tissue sections, and on to ultrastructural levels. The step-by-step protocols used reflect research in these areas and are all reproducible.
Chapter 1. Genomic <i>In Situ</i> Hybridization for Whole Chromosome and Genome Analysis (K. Anamthawat-Jósson, <i>et al.</i>). <p>Chapter 2. Fluorescence <i>In Situ</i> Hybridization: Applications in Gene Mapping and Clinical Diagnostics (G. A. Evans and L. Selleri).</p> <p>Chapter 3. Detection of Nucleic Acids (DNA and RNA) <i>In Situ</i> by Single and Cyclic Primed <i>In Situ</i> Labelling (PRINS): Two Alternatives to Traditional <i>In Situ</i> Hybridization Methods (J. Hindkjær, <i>et al.</i>).</p> <p>Chapter 4. Fluorescence Immunophenotyping and Interphase Cytogenetics as a Tool for Investigation of Neoplasms (FICTION): Combined <i>In Situ</i> Hybridization and Fluorescence Immunophenotyping (K. Weber-Matthiesen).</p> <p>Chapter 5. <i>In Situ</i> Hybridization to Embryo Whole Mounts and Tissue Sections: mRNA Detection and Application to Developmental Studies (T. Jowett, <i>et al.</i>).</p> <p>Chapter 6. <i>In Situ</i> Hybridization at the Electron Microscopic Level (F. Wachtler, <i>et al.</i>).</p> <p>Appendix I: Labelling DNA Probes for <i>In Situ</i> Hybridization.</p> <p>Appendix II. Quantification of Efficiency of Incorporation of Labelled Nucleotides.</p> <p>Glossary.</p> <p>Subject Index.</p>
<p>Melody Clark is the author of In Situ Hybridization: Laboratory Companion, published by Wiley.
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